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mouse monoclonal antiflag peroxidase (hrp) igg  (Millipore)

 
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    Millipore mouse monoclonal antiflag peroxidase (hrp) igg
    PEGylation of bispecific S-Fab using 20 kDa linear MAL-PEG-OMe. Notes: S-Fab was reacted with the functionalized PEG at a series of ratios (0:1, 10:1, 20:1, 40:1, and 60:1 molar equivalents of PEG:S-Fab). After shaking at 22°C for 2 h, the samples were examined by reducing SDS-PAGE (5 μL per sample). ( A ) Coomassie blue staining of S-Fab with different molar ratios of PEG:S-Fab during PEGylation. ( B ) Barium iodide complex dye staining for S-Fab with different molar ratios of PEG:S-Fab. ( C ) Western blotting assay using an <t>antiflag</t> antibody to detect the VH-CH-CEA chain. ( D ) Western blotting assay using an anti-His6 antibody to detect the VL-CL chain. Numbers 0, 10, 20, 40, and 60 represent the molar ratio of PEG:Fab at 0:1, 10:1, 20:1, 40:1, and 60:1, respectively. M (kDa), molecular weight markers (kilodalton). Abbreviations: CEA, carcinoembryonic antigen; MAL-PEG-OMe, methoxy PEG maleimide; PEG, polyethylene glycol; S-Fab, single-domain antibody-linked Fab; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis.
    Mouse Monoclonal Antiflag Peroxidase (Hrp) Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal antiflag peroxidase (hrp) igg/product/Millipore
    Average 90 stars, based on 1 article reviews
    mouse monoclonal antiflag peroxidase (hrp) igg - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "Site-specific PEGylation of an anti-CEA/CD3 bispecific antibody improves its antitumor efficacy"

    Article Title: Site-specific PEGylation of an anti-CEA/CD3 bispecific antibody improves its antitumor efficacy

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S164542

    PEGylation of bispecific S-Fab using 20 kDa linear MAL-PEG-OMe. Notes: S-Fab was reacted with the functionalized PEG at a series of ratios (0:1, 10:1, 20:1, 40:1, and 60:1 molar equivalents of PEG:S-Fab). After shaking at 22°C for 2 h, the samples were examined by reducing SDS-PAGE (5 μL per sample). ( A ) Coomassie blue staining of S-Fab with different molar ratios of PEG:S-Fab during PEGylation. ( B ) Barium iodide complex dye staining for S-Fab with different molar ratios of PEG:S-Fab. ( C ) Western blotting assay using an antiflag antibody to detect the VH-CH-CEA chain. ( D ) Western blotting assay using an anti-His6 antibody to detect the VL-CL chain. Numbers 0, 10, 20, 40, and 60 represent the molar ratio of PEG:Fab at 0:1, 10:1, 20:1, 40:1, and 60:1, respectively. M (kDa), molecular weight markers (kilodalton). Abbreviations: CEA, carcinoembryonic antigen; MAL-PEG-OMe, methoxy PEG maleimide; PEG, polyethylene glycol; S-Fab, single-domain antibody-linked Fab; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis.
    Figure Legend Snippet: PEGylation of bispecific S-Fab using 20 kDa linear MAL-PEG-OMe. Notes: S-Fab was reacted with the functionalized PEG at a series of ratios (0:1, 10:1, 20:1, 40:1, and 60:1 molar equivalents of PEG:S-Fab). After shaking at 22°C for 2 h, the samples were examined by reducing SDS-PAGE (5 μL per sample). ( A ) Coomassie blue staining of S-Fab with different molar ratios of PEG:S-Fab during PEGylation. ( B ) Barium iodide complex dye staining for S-Fab with different molar ratios of PEG:S-Fab. ( C ) Western blotting assay using an antiflag antibody to detect the VH-CH-CEA chain. ( D ) Western blotting assay using an anti-His6 antibody to detect the VL-CL chain. Numbers 0, 10, 20, 40, and 60 represent the molar ratio of PEG:Fab at 0:1, 10:1, 20:1, 40:1, and 60:1, respectively. M (kDa), molecular weight markers (kilodalton). Abbreviations: CEA, carcinoembryonic antigen; MAL-PEG-OMe, methoxy PEG maleimide; PEG, polyethylene glycol; S-Fab, single-domain antibody-linked Fab; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis.

    Techniques Used: SDS Page, Staining, Western Blot, Molecular Weight, Polyacrylamide Gel Electrophoresis

    Pharmacokinetic ( A and B ) and stability ( C – F ) analyses of the serum concentrations of PEG-S-Fab and S-Fab. Notes: ( A ) Standard curve for the ELISA. ( B ) Serum PEG-S-Fab concentration–time curve after intravenous administration. Each data point is expressed as the mean ± SEM. Western blotting assay to detect the heavy chain of resulting S-Fab ( C ) or PEG-S-Fab ( D ) using an antiflag antibody after incubation with human plasma. Western blotting assay to detect the light chain of resulting S-Fab ( E ) or PEG-S-Fab ( F ) using an anti-His antibody after incubation with human plasma. M (kDa), molecular weight markers (kilodalton); P, plasma only group. Abbreviations: PEG, polyethylene glycol; PEG-S-Fab, PEGylated S-Fab; S-Fab, single-domain antibody-linked Fab; SEM, standard error of the mean; ELISA, enzyme-linked immunosorbent assay.
    Figure Legend Snippet: Pharmacokinetic ( A and B ) and stability ( C – F ) analyses of the serum concentrations of PEG-S-Fab and S-Fab. Notes: ( A ) Standard curve for the ELISA. ( B ) Serum PEG-S-Fab concentration–time curve after intravenous administration. Each data point is expressed as the mean ± SEM. Western blotting assay to detect the heavy chain of resulting S-Fab ( C ) or PEG-S-Fab ( D ) using an antiflag antibody after incubation with human plasma. Western blotting assay to detect the light chain of resulting S-Fab ( E ) or PEG-S-Fab ( F ) using an anti-His antibody after incubation with human plasma. M (kDa), molecular weight markers (kilodalton); P, plasma only group. Abbreviations: PEG, polyethylene glycol; PEG-S-Fab, PEGylated S-Fab; S-Fab, single-domain antibody-linked Fab; SEM, standard error of the mean; ELISA, enzyme-linked immunosorbent assay.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Concentration Assay, Western Blot, Incubation, Molecular Weight



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    PEGylation of bispecific S-Fab using 20 kDa linear MAL-PEG-OMe. Notes: S-Fab was reacted with the functionalized PEG at a series of ratios (0:1, 10:1, 20:1, 40:1, and 60:1 molar equivalents of PEG:S-Fab). After shaking at 22°C for 2 h, the samples were examined by reducing SDS-PAGE (5 μL per sample). ( A ) Coomassie blue staining of S-Fab with different molar ratios of PEG:S-Fab during PEGylation. ( B ) Barium iodide complex dye staining for S-Fab with different molar ratios of PEG:S-Fab. ( C ) Western blotting assay using an <t>antiflag</t> antibody to detect the VH-CH-CEA chain. ( D ) Western blotting assay using an anti-His6 antibody to detect the VL-CL chain. Numbers 0, 10, 20, 40, and 60 represent the molar ratio of PEG:Fab at 0:1, 10:1, 20:1, 40:1, and 60:1, respectively. M (kDa), molecular weight markers (kilodalton). Abbreviations: CEA, carcinoembryonic antigen; MAL-PEG-OMe, methoxy PEG maleimide; PEG, polyethylene glycol; S-Fab, single-domain antibody-linked Fab; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis.
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    Image Search Results


    PEGylation of bispecific S-Fab using 20 kDa linear MAL-PEG-OMe. Notes: S-Fab was reacted with the functionalized PEG at a series of ratios (0:1, 10:1, 20:1, 40:1, and 60:1 molar equivalents of PEG:S-Fab). After shaking at 22°C for 2 h, the samples were examined by reducing SDS-PAGE (5 μL per sample). ( A ) Coomassie blue staining of S-Fab with different molar ratios of PEG:S-Fab during PEGylation. ( B ) Barium iodide complex dye staining for S-Fab with different molar ratios of PEG:S-Fab. ( C ) Western blotting assay using an antiflag antibody to detect the VH-CH-CEA chain. ( D ) Western blotting assay using an anti-His6 antibody to detect the VL-CL chain. Numbers 0, 10, 20, 40, and 60 represent the molar ratio of PEG:Fab at 0:1, 10:1, 20:1, 40:1, and 60:1, respectively. M (kDa), molecular weight markers (kilodalton). Abbreviations: CEA, carcinoembryonic antigen; MAL-PEG-OMe, methoxy PEG maleimide; PEG, polyethylene glycol; S-Fab, single-domain antibody-linked Fab; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis.

    Journal: International Journal of Nanomedicine

    Article Title: Site-specific PEGylation of an anti-CEA/CD3 bispecific antibody improves its antitumor efficacy

    doi: 10.2147/IJN.S164542

    Figure Lengend Snippet: PEGylation of bispecific S-Fab using 20 kDa linear MAL-PEG-OMe. Notes: S-Fab was reacted with the functionalized PEG at a series of ratios (0:1, 10:1, 20:1, 40:1, and 60:1 molar equivalents of PEG:S-Fab). After shaking at 22°C for 2 h, the samples were examined by reducing SDS-PAGE (5 μL per sample). ( A ) Coomassie blue staining of S-Fab with different molar ratios of PEG:S-Fab during PEGylation. ( B ) Barium iodide complex dye staining for S-Fab with different molar ratios of PEG:S-Fab. ( C ) Western blotting assay using an antiflag antibody to detect the VH-CH-CEA chain. ( D ) Western blotting assay using an anti-His6 antibody to detect the VL-CL chain. Numbers 0, 10, 20, 40, and 60 represent the molar ratio of PEG:Fab at 0:1, 10:1, 20:1, 40:1, and 60:1, respectively. M (kDa), molecular weight markers (kilodalton). Abbreviations: CEA, carcinoembryonic antigen; MAL-PEG-OMe, methoxy PEG maleimide; PEG, polyethylene glycol; S-Fab, single-domain antibody-linked Fab; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis.

    Article Snippet: The goat antihuman IgG (H + L)-AlexaFluor 488 antibody (Lot A11001; Thermo Fisher Scientific), mouse monoclonal antiflag peroxidase (HRP) IgG (Lot A8492; Sigma-Aldrich Co., St Louis, MO, USA), mouse monoclonal anti-His IgG (Lot 14-6657-82; Thermo Fisher Scientific), and goat antimouse HRP-conjugated IgG (Lot ab97040; Abcam Co., Ltd., Shanghai, People’s Republic of China) were purchased accordingly.

    Techniques: SDS Page, Staining, Western Blot, Molecular Weight, Polyacrylamide Gel Electrophoresis

    Pharmacokinetic ( A and B ) and stability ( C – F ) analyses of the serum concentrations of PEG-S-Fab and S-Fab. Notes: ( A ) Standard curve for the ELISA. ( B ) Serum PEG-S-Fab concentration–time curve after intravenous administration. Each data point is expressed as the mean ± SEM. Western blotting assay to detect the heavy chain of resulting S-Fab ( C ) or PEG-S-Fab ( D ) using an antiflag antibody after incubation with human plasma. Western blotting assay to detect the light chain of resulting S-Fab ( E ) or PEG-S-Fab ( F ) using an anti-His antibody after incubation with human plasma. M (kDa), molecular weight markers (kilodalton); P, plasma only group. Abbreviations: PEG, polyethylene glycol; PEG-S-Fab, PEGylated S-Fab; S-Fab, single-domain antibody-linked Fab; SEM, standard error of the mean; ELISA, enzyme-linked immunosorbent assay.

    Journal: International Journal of Nanomedicine

    Article Title: Site-specific PEGylation of an anti-CEA/CD3 bispecific antibody improves its antitumor efficacy

    doi: 10.2147/IJN.S164542

    Figure Lengend Snippet: Pharmacokinetic ( A and B ) and stability ( C – F ) analyses of the serum concentrations of PEG-S-Fab and S-Fab. Notes: ( A ) Standard curve for the ELISA. ( B ) Serum PEG-S-Fab concentration–time curve after intravenous administration. Each data point is expressed as the mean ± SEM. Western blotting assay to detect the heavy chain of resulting S-Fab ( C ) or PEG-S-Fab ( D ) using an antiflag antibody after incubation with human plasma. Western blotting assay to detect the light chain of resulting S-Fab ( E ) or PEG-S-Fab ( F ) using an anti-His antibody after incubation with human plasma. M (kDa), molecular weight markers (kilodalton); P, plasma only group. Abbreviations: PEG, polyethylene glycol; PEG-S-Fab, PEGylated S-Fab; S-Fab, single-domain antibody-linked Fab; SEM, standard error of the mean; ELISA, enzyme-linked immunosorbent assay.

    Article Snippet: The goat antihuman IgG (H + L)-AlexaFluor 488 antibody (Lot A11001; Thermo Fisher Scientific), mouse monoclonal antiflag peroxidase (HRP) IgG (Lot A8492; Sigma-Aldrich Co., St Louis, MO, USA), mouse monoclonal anti-His IgG (Lot 14-6657-82; Thermo Fisher Scientific), and goat antimouse HRP-conjugated IgG (Lot ab97040; Abcam Co., Ltd., Shanghai, People’s Republic of China) were purchased accordingly.

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Western Blot, Incubation, Molecular Weight

    Journal: eLife

    Article Title: A missense in HSF2BP causing primary ovarian insufficiency affects meiotic recombination by its novel interactor C19ORF57/BRME1

    doi: 10.7554/eLife.56996

    Figure Lengend Snippet:

    Article Snippet: The primary antibodies used for immunofluorescence were rabbit αHSF2BP R2 (1:30, ProteintechTM), rabbit αBRME1 R2 (1:100, ProteintechTM), mouse αSYCP3 IgG sc-74569 (1:100, Santa Cruz), rabbit α-SYCP3 serum K921 (provided by Dr. José Luis Barbero, Centro de Investigaciones Biológicas, Spain), rabbit αSYCP1 IgG ab15090 (1:200, Abcam), rabbit anti-γH2AX (ser139) IgG #07–164 (1:500, Millipore), mouse αMLH1 51-1327GR (1:20, BD Biosciences), mouse αCDK2 (1:20; Santa Cruz Sc-6248) rabbit αRAD51 PC130 (1:50, Calbiochem), rabbit αRPA1 serum ¨Molly¨ (1:30, provided by Dr. Edyta Marcon, Medical Research University of Toronto, Canada), rat αRPA2 2208S (1:100, Cell Signaling), rabbit αDMC1 (1:500, ProteintechTM), rabbit αSPATA22 16989–1-AP (1:60, Proteintech), mouse αFlag IgG (1:100; F1804, Sigma-Aldrich).

    Techniques: Recombinant, Generated, Sequencing, CRISPR, DNA Binding Assay, In Situ, cDNA Library Assay, Staining, Plasmid Preparation